Posts Tagged ‘hplc calibration’
Flow Meter Calibration for HPLC Pumping Systems
Calibrating digital liquid flow meters that test high performance liquid chromatography (HPLC) pumping systems is performed prior to units being put into service. In accordance with GLP and to comply with other regulations, flow meter calibration – recalibration – should be performed annually. Digital HPLC flow meter recalibration is an exacting procedure requiring experts using high-precision measuring instruments. We describe it in this post after a brief explanation on
What Flow Meters Meter
As the name implies, flow meters measure the rate of flow of a product, usually through a pipeline or processing line. Gasoline pump flow meters at your local filling station are another common example. Standards may apply to periodic certification by calibrating flow meters to confirm that you are getting the fuel you pay for. Heating oil, natural or propane gas are also delivered via flow meters that vendors use to calculate your bill.
As another example, … Read the rest
How a HPLC Digital Flow Meter Works
Before explaining how a HPLC digital flow meter works here’s an explanation of what digital flow meters do:
Digital flowmeters performing high performance liquid chromatography are used to measure the accuracy of HPLC pumping systems in order to meet FDA and other regulations regarding installation qualification (IQ), operational qualification (OQ) and performance qualification (PQ) of equipment used in the pharmaceutical and biopharmaceutical industries as well as other analytical labs.
The process demands accurate, reproducible column retention time as an analyte (what is being analyzed) is pumped through the HPLC system.
These precision digital flow meters are not to be likened to flow meters used on process lines measuring flow rates in, for example, thousands of gallons per minute but instead in fractions of milliliters per minute. … Read the rest
How to Check Pump System Accuracy for HPLC Analysis
Pump system accuracy is crucial for equipment used to conduct high performance liquid chromatography (HPLC) analysis in the pharmaceutical and other industries subject to regulatory oversight by the FDA and similar authorities. Among functions performed by HPLC are included checks for purity, monitoring changes in pharmaceutical intermediates during chemical synthesis scale-up, stability and dissolution tests, and performing ongoing quality control procedures.
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A Brief on HPLC Analysis
Identifying an analyte by HPLC is based on accurate and reproducible column retention time as the analyte is pumped through an HPLC system. Reproducible retention time is critical and depends on accurate, uniform, reproducible HPLC pump flow rates.
Note that it is important to distinguish between accuracy and precision. These two terms may seem the same but they are not necessarily so. For example, an HPLC system (or for that matter many other systems such as analytical scales) may … Read the rest
