Posts Tagged ‘hplc sample preparation’

Sample preparation to transform analytes into measurable form can involve complex processes. It is important that the transformation or extraction process avoids chemical degradation that can be caused by excessive heat or mechanically induced damage.

Ultrasonic energy is a proven technique to achieve fast, safe sample preparation.  As an example it is a method often specified in USP monographs to extract active pharmaceutical ingredients from carriers for content uniformity and potency assay tests. The ultrasonic process is also ideal for dissolving, dispersing, emulsifying, homogenizing and mixing.

Sample Preparation Steps

Samples to be analyzed are placed in flasks along with a recommended solvent.  Flasks are partially immersed in a sonicator bath containing a surfactant.   The 37 kHz Elmasonic S Select 150 ultrasonic bath available from Tovatech is especially designed to quickly and safely accomplish sample preparation.  The inside dimensions of the shallow basket, (LxWxH) 17.9 x 9.8 x 2.2 … Read the rest